A Determination of Valosin-Containing Protein (p97/VCP) and Small VCP-Interacting Protein (SVIP) Expressions in Rat Kidney and Vero Cells

Abstract

Objective: The 97-kDa valosin-containing protein (p97/VCP), which is involved in the ubiquitin-proteasome-mediated degradation pathway, regulates a diversity of cellular activities. p97/VCP, which also physically interacts with the 26 proteasome and uses multiubiquitin chains to immediately attach the ubiquitinated substrates. Small p97/VCP-interacting protein (SVIP), was first detected to be one of numerous cofactors controlling the VCP. In rat kidney tissues and Vero cells, SVIP and p97/VCP proteins haven't been investigated. The aim of present study was to examine the expression of p97/VCP and SVIP in kidney tissues and Vero cells. Also, this study was purposed to conduce to further research on the use of two proteins as kidney cell biomarkers in cases of renal pathologies. Methods: Hematoxylin-eosin (HE) staining was used to examine the histomorphology of rat kidney tissues. The cellular distribution and expression of SVIP and p97/VCP in rat renal tissue were investigated using the immunohistochemistry method. Also, Vero kidney cell line was used to determine cellular localization and expression of ERAD proteins by immunofluorescence and Western blotting. Results: Immunohistochemistry analysis revealed that SVIP and p97/VCP were primarily located in the nucleus and cytoplasm of the proximal and distal tubule cells in the rat kidney. Furthermore, the immunoexpression of these proteins was discovered in the epithelial cells of the Henle loop and collecting tubules in the medulla. In the nucleus and cytoplasm of Vero kidney cells in vitro, p97/VCP immunoexpression was detected. SVIP was determined in the cytoplasm of the cells. Also, Vero cells showed intense p97/VCP expression compared to SVIP expression by Western blotting. Conclusions: In conclusion, the current study showed that p97/VCP and SVIP had cellular localization and protein expression in the rat kidney tissue and kidney cell line (Vero). We might advise that additional research is required to ascertain the role of SVIP and p97/VCP in renal tissue.